Hotspot

psbA-trnK-UUU

LSC · 1145-1380 · 236 bp

Back to Eleusine

Pi 0

MarkerSeek score 29.00

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 236
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	AACACCAAACCATCCGATGT	GCACGATTTGGGGAGAGATT	45.0	50.0	57.7	58.0	434 bp	1.0	77.4
psbA-trnK-UUU_p2	GCTTGTACTTTCGCGTCTCT	GCACGATTTGGGGAGAGATT	50.0	50.0	58.3	58.0	365 bp	1.0	77.2
psbA-trnK-UUU_p3	AACACCAAACCATCCGATGT	AAATGCAAGCACGATTTGGG	45.0	45.0	57.7	57.9	442 bp	1.0	77.0
psbA-trnK-UUU_p4	GCTTGTACTTTCGCGTCTCT	AAATGCAAGCACGATTTGGG	50.0	45.0	58.3	57.9	373 bp	1.0	76.9
psbA-trnK-UUU_p5	CCCCACAGGCTTGTACTTTC	GCACGATTTGGGGAGAGATT	55.0	50.0	58.5	58.0	373 bp	1.0	75.9