Hotspot

trnE-UUC-rpoB

LSC · 11994-12813 · 820 bp

Back to Elephantopus

Pi 0
MarkerSeek score 40.00
Primer available yes
Label trnE-UUC-rpoB

Feature Metadata

label_name
trnE-UUC-rpoB
feature_type
igs
region
LSC
length_bp
820
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-rpoB_p1 GGGACTTGAACCCTCGTTTT AGTTCTTCTGTCAAACCCTGA 50.0 42.9 58.0 56.8 962 bp 1.0 62.1
trnE-UUC-rpoB_p2 TAACCACTGGACCATAGGGG AGTTCTTCTGTCAAACCCTGA 55.0 42.9 58.1 56.8 921 bp 1.0 61.4
trnE-UUC-rpoB_p3 CCCTCGTTTTCTCCGTGAAA AGTTCTTCTGTCAAACCCTGA 50.0 42.9 58.1 56.8 952 bp 1.0 61.3
trnE-UUC-rpoB_p4 TTGAACCCTCGTTTTCTCCG AGTTCTTCTGTCAAACCCTGA 50.0 42.9 58.1 56.8 957 bp 1.0 61.3
trnE-UUC-rpoB_p5 GGGACTTGAACCCTCGTTTT TCAAACCCTGATCAATGAACCT 50.0 40.9 58.0 57.6 952 bp 1.0 60.7