Hotspot

rps16-trnQ-UUG

LSC · 6232-7159 · 928 bp

Back to Elephantopus

Pi 0

MarkerSeek score 40.00

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 928
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1001 bp	1.0	78.3
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	TAAGGCATCGGGTTTTGGTC	45.0	50.0	58.1	58.2	1031 bp	1.0	77.5
rps16-trnQ-UUG_p3	CGGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1016 bp	1.0	76.4
rps16-trnQ-UUG_p4	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1015 bp	1.0	76.4
rps16-trnQ-UUG_p5	GGATCGTGTCCTTCAAGTCG	TAAGGCATCGGGTTTTGGTC	55.0	50.0	58.4	58.2	1045 bp	1.0	75.7