Hotspot

trnK-UUU-rps16

LSC · 4382-5098 · 717 bp

Back to Elephantopus

Pi 0
MarkerSeek score 40.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
717
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT CCTTGAAAAAGGTGCTCAGC 50.0 50.0 58.2 57.6 863 bp 1.0 75.2
trnK-UUU-rps16_p2 AAAGCCGAGTACTCTACCGT CTTGAAAAAGGTGCTCAGCC 50.0 50.0 58.2 57.6 862 bp 1.0 75.2
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT CCTTGAAAAAGGTGCTCAGC 50.0 50.0 58.2 57.6 862 bp 1.0 75.2
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT CTTGAAAAAGGTGCTCAGCC 50.0 50.0 58.2 57.6 861 bp 1.0 75.2
trnK-UUU-rps16_p5 TCAGTCGTGGTCTTCCAAAC CTTGAAAAAGGTGCTCAGCC 50.0 50.0 57.8 57.6 947 bp 1.0 74.7