Hotspot

rpl32-trnL-UAG

SSC · 115010-115967 · 958 bp

Back to Elatostematoides

Pi 0.00532

MarkerSeek score 61.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 958
variable_sites: 5
pi: 0.00532
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGAAAGGATATTGGGCAGCG	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	1063-1082 bp	1.0	81.5
rpl32-trnL-UAG_p2	AGAAAGGATATTGGGCAGCG	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	1064-1083 bp	1.0	81.5
rpl32-trnL-UAG_p3	AGAAAGGATATTGGGCAGCG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	1073-1092 bp	1.0	80.6
rpl32-trnL-UAG_p4	AAAGGATATTGGGCAGCGTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.9	58.0	1061-1080 bp	1.0	80.6
rpl32-trnL-UAG_p5	AAAGGATATTGGGCAGCGTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.9	58.0	1062-1081 bp	1.0	80.6