Hotspot

petA-psbJ

LSC · 64331-65284 · 954 bp

Back to Elatostematoides

Pi 0.00629
MarkerSeek score 62.40
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
954
variable_sites
6
pi
0.00629
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 ATTACGCGTACAAGGCCTTT GCCGATACTACTGGAAGGAT 45.0 50.0 57.9 56.2 1189-1205 bp 1.0 65.2
petA-psbJ_p2 TTACGCGTACAAGGCCTTTT GCCGATACTACTGGAAGGAT 45.0 50.0 58.1 56.2 1188-1204 bp 1.0 65.1
petA-psbJ_p3 ATTACGCGTACAAGGCCTTT GCCGATACTACTGGAAGGATT 45.0 47.6 57.9 57.0 1189-1205 bp 1.0 63.3
petA-psbJ_p4 TTACGCGTACAAGGCCTTTT GCCGATACTACTGGAAGGATT 45.0 47.6 58.1 57.0 1188-1204 bp 1.0 63.2
petA-psbJ_p5 ACAAGGCCTTTTGCTCTTCT GCCGATACTACTGGAAGGAT 45.0 50.0 57.6 56.2 1180-1196 bp 1.0 63.1