Hotspot

accD-psaI

LSC · 59842-60512 · 671 bp

Back to Elatostematoides

Pi 0.00304

MarkerSeek score 62.40

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 671
variable_sites: 2
pi: 0.00304
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TTGATCCAATCGTACCACGT	AAACCATAGCAATTGCCGGA	45.0	45.0	57.0	58.2	850-863 bp	1.0	71.8
accD-psaI_p2	TTGATCCAATCGTACCACGT	AACCATAGCAATTGCCGGAA	45.0	45.0	57.0	58.2	849-862 bp	1.0	71.8
accD-psaI_p3	TTGATCCAATCGTACCACGT	ACCATAGCAATTGCCGGAAA	45.0	45.0	57.0	58.2	848-861 bp	1.0	71.8
accD-psaI_p4	TTGATCCAATCGTACCACGT	GAAACCATAGCAATTGCCGG	45.0	50.0	57.0	57.8	851-864 bp	1.0	71.4
accD-psaI_p5	TTGATCCAATCGTACCACGT	GCGGGTAAGTTGCTGATAGT	45.0	50.0	57.0	57.7	800-813 bp	1.0	70.7