Hotspot

trnL-UAA-trnF-GAA

LSC · 48792-49181 · 390 bp

Back to Elatostematoides

Pi 0.0181

MarkerSeek score 58.30

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 390
variable_sites: 7
pi: 0.0181
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AATCGTGAGGGTTCAAGTCC	TCTACCAGCTGAGCTATCCC	50.0	55.0	57.5	58.0	441-444 bp	1.0	78.0
trnL-UAA-trnF-GAA_p2	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	477-480 bp	1.0	77.5
trnL-UAA-trnF-GAA_p3	AATCGTGAGGGTTCAAGTCC	GTGCCAGGAACCAGATTTGA	50.0	50.0	57.5	58.1	492-495 bp	1.0	77.1
trnL-UAA-trnF-GAA_p4	AATCGTGAGGGTTCAAGTCC	TCAGTCCTCTGCTCTACCAG	50.0	55.0	57.5	57.9	453-456 bp	1.0	76.9
trnL-UAA-trnF-GAA_p5	AATCGTGAGGGTTCAAGTCC	GTGTGCCAGGAACCAGATTT	50.0	50.0	57.5	58.4	494-497 bp	1.0	74.8