Hotspot

trnS-GCU-trnG-UCC

LSC · 8613-9346 · 734 bp

Back to Elatostematoides

Pi 0.00596
MarkerSeek score 65.90
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
734
variable_sites
4
pi
0.00596
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.91
markerseek_score
65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA AAGCTTTTGATCGGACTGGG 50.0 50.0 58.0 58.2 813-877 bp 1.0 83.0
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC AAGCTTTTGATCGGACTGGG 50.0 50.0 58.0 58.2 812-876 bp 1.0 82.9
trnS-GCU-trnG-UCC_p3 ACGGAAAGAGAGGGATTCGA AAGCTTTTGATCGGACTGGG 50.0 50.0 58.2 58.2 867-931 bp 1.0 81.8
trnS-GCU-trnG-UCC_p4 GTCCACTCAGCCATCTTTCC AAGCTTTTGATCGGACTGGG 55.0 50.0 58.3 58.2 798-862 bp 1.0 80.9
trnS-GCU-trnG-UCC_p5 AGCAATCCGACGCTTTAGTC AAGCTTTTGATCGGACTGGG 50.0 50.0 58.4 58.2 815-879 bp 1.0 80.1