Hotspot

trnK-UUU-rps16

LSC · 4364-5192 · 829 bp

Back to Elatostematoides

Pi 0.00494
MarkerSeek score 63.80
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
829
variable_sites
4
pi
0.00494
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AGTCGCGGTCTTACAAACTC ATTTCAAAGAAGGCGGGTGT 50.0 45.0 57.9 58.0 994-1001 bp 1.0 81.3
trnK-UUU-rps16_p2 GTCGCGGTCTTACAAACTCT ATTTCAAAGAAGGCGGGTGT 50.0 45.0 57.9 58.0 993-1000 bp 1.0 81.3
trnK-UUU-rps16_p3 AGTCGCGGTCTTACAAACTC AAAAGGCGCTCATCCTACAG 50.0 50.0 57.9 58.0 1028-1035 bp 1.0 81.1
trnK-UUU-rps16_p4 GTCGCGGTCTTACAAACTCT AAAAGGCGCTCATCCTACAG 50.0 50.0 57.9 58.0 1027-1034 bp 1.0 81.1
trnK-UUU-rps16_p5 GTCGCGGTCTTACAAACTCT TTGAAAAAGGCGCTCATCCT 50.0 45.0 57.9 58.1 1031-1038 bp 1.0 80.6