Hotspot

ycf1

SSC · 123751-129321 · 5571 bp

Back to Elatostema

Pi 0.082

MarkerSeek score 72.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5571
variable_sites: 1662
pi: 0.082
unique_haplotype_count: 42
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TAGCATACCGAAACGACTGC	AGCTCGTACCACAAGAGAGT	50.0	50.0	58.1	58.1	1334-1376 bp	0.232558	59.0
ycf1_p2	TGCTGTGATTAACGAGCGAA	AGCTCGTACCACAAGAGAGT	45.0	50.0	57.9	58.1	990 bp	0.069767	41.5
ycf1_p3	TTGCTGTGATTAACGAGCGA	AGCTCGTACCACAAGAGAGT	45.0	50.0	57.9	58.1	991 bp	0.069767	41.5
ycf1_p4	TGCTGTGATTAACGAGCGAA	TCAGCTCGTACCACAAGAGA	45.0	50.0	57.9	58.1	992 bp	0.069767	41.5
ycf1_p5	TTGCTGTGATTAACGAGCGA	TCAGCTCGTACCACAAGAGA	45.0	50.0	57.9	58.1	993 bp	0.069767	41.5