Hotspot

petA-psbJ

LSC · 63509-64423 · 915 bp

Back to Elatostema

Pi 0.0449
MarkerSeek score 82.70
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
915
variable_sites
218
pi
0.0449
unique_haplotype_count
39
primer_available
yes
alignment_reliability
0.98
markerseek_score
82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 AGAAACAGTTTGAGAAGGTTCA TTGTGATCGGTTTAATCGGT 36.4 40.0 55.8 55.1 713-1082 bp 1.0 52.0
petA-psbJ_p2 AGAAACAGTTTGAGAAGGTTCA TCTGGATTAGGTTCATCCCT 36.4 45.0 55.8 54.9 672-1041 bp 1.0 52.0
petA-psbJ_p3 AAGAAACAGTTTGAGAAGGTTCA TTGTGATCGGTTTAATCGGT 34.8 40.0 56.5 55.1 714-1083 bp 1.0 52.0
petA-psbJ_p4 AGAAACAGTTTGAGAAGGTTCAA TTGTGATCGGTTTAATCGGT 34.8 40.0 56.5 55.1 713-1082 bp 1.0 52.0
petA-psbJ_p5 AGAAACAGTTTGAGAAGGTTCA TCTGGATTAGGTTCATCCCTT 36.4 42.9 55.8 55.7 672-1041 bp 1.0 52.0