Hotspot

ycf4-cemA

LSC · 60908-61648 · 741 bp

Back to Elatostema

Pi 0.0357

MarkerSeek score 77.30

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 741
variable_sites: 168
pi: 0.0357
unique_haplotype_count: 41
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCCTATTTCTTGCGTGT	AGGGAAATAAATGCTTTCTTTTT	45.0	26.1	58.1	53.3	449-825 bp	1.0	52.0
ycf4-cemA_p2	TGAACAGAAAGCCGCTGAAT	AGGGAAATAAATGCTTTCTTTTT	45.0	26.1	58.1	53.3	468-844 bp	1.0	52.0
ycf4-cemA_p3	TTGGCCTATTTCTTGCGTGT	AGGGAAATAAATGCTTTCTTTT	45.0	27.3	58.1	52.5	449-825 bp	0.976744	51.1
ycf4-cemA_p4	ATTGAACAGAAAGCCGCTGA	AGGGAAATAAATGCTTTCTTTT	45.0	27.3	58.1	52.5	470-846 bp	0.976744	51.1
ycf4-cemA_p5	TGAACAGAAAGCCGCTGAAT	AGGGAAATAAATGCTTTCTTTT	45.0	27.3	58.1	52.5	468-844 bp	0.976744	51.1