Hotspot

psbZ-trnG-GCC

LSC · 36031-36544 · 514 bp

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Pi 0.0424
MarkerSeek score 79.80
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
514
variable_sites
126
pi
0.0424
unique_haplotype_count
39
primer_available
yes
alignment_reliability
0.92
markerseek_score
79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 TCTGGTACAACATTATGGATTGGA AATCGAACCCGCATCTTCTC 37.5 50.0 57.7 58.1 598-664 bp 1.0 57.0
psbZ-trnG-GCC_p2 TCTGGTACAACATTATGGATTGGA GAATCGAACCCGCATCTTCT 37.5 50.0 57.7 58.1 599-665 bp 1.0 57.0
psbZ-trnG-GCC_p3 TCTGGTACAACATTATGGATTGGA CATCTTCTCCGTGGCAAAGA 37.5 50.0 57.7 57.9 587-653 bp 1.0 56.6
psbZ-trnG-GCC_p4 TCTGGTACAACATTATGGATTGGA CTTCTCCGTGGCAAAGAGAA 37.5 50.0 57.7 57.8 584-650 bp 1.0 56.1
psbZ-trnG-GCC_p5 TCTGGTACAACATTATGGATTGGA ATCTTCTCCGTGGCAAAGAG 37.5 50.0 57.7 57.6 586-652 bp 1.0 54.3