Hotspot

atpB-rbcL

LSC · 133652-134299 · 648 bp

Back to Elaphoglossum

Pi 0.0144

MarkerSeek score 65.70

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 648
variable_sites: 14
pi: 0.0144
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CACATCCAACACTGGTCCAA	GCTTTGAATCCAACACCTGC	50.0	50.0	58.0	57.9	780-786 bp	1.0	81.3
atpB-rbcL_p2	CACATCCAACACTGGTCCAA	GTCTCCGTTTGTGGTGACAT	50.0	50.0	58.0	58.1	756-762 bp	1.0	81.0
atpB-rbcL_p3	CACATCCAACACTGGTCCAA	TTGGTCTTGTACTCAGGGGT	50.0	50.0	58.0	57.9	831-837 bp	1.0	80.9
atpB-rbcL_p4	CACATCCAACACTGGTCCAA	AACACCTGCTTTAGTCTCCG	50.0	50.0	58.0	57.5	769-775 bp	1.0	78.3
atpB-rbcL_p5	CACATCCAACACTGGTCCAA	CACCTGCTTTAGTCTCCGTT	50.0	50.0	58.0	57.5	767-773 bp	1.0	78.3