Hotspot

ndhC-trnM-CAU

LSC · 131147-131537 · 391 bp

Back to Elaphoglossum

Pi 0.0309

MarkerSeek score 65.90

Primer available yes

Label ndhC-trnM-CAU

Feature Metadata

label_name: ndhC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 391
variable_sites: 17
pi: 0.0309
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnM-CAU_p1	GGAGTCATATTGGTGGAGCG	GCGATACTCTAACCGCTGAG	55.0	55.0	58.1	58.0	439-454 bp	1.0	84.4
ndhC-trnM-CAU_p2	GGAGTCATATTGGTGGAGCG	TTCGAACCAATGACTCTCGC	55.0	50.0	58.1	58.3	468-483 bp	1.0	82.0
ndhC-trnM-CAU_p3	GGAGTCATATTGGTGGAGCG	CAATGACTCTCGCCGTATGA	55.0	50.0	58.1	57.5	461-476 bp	1.0	80.5
ndhC-trnM-CAU_p4	GGAGTCATATTGGTGGAGCG	CCAATGACTCTCGCCGTATG	55.0	55.0	58.1	58.5	462-477 bp	1.0	80.4
ndhC-trnM-CAU_p5	GGAGTCATATTGGTGGAGCG	TGACTCTCGCCGTATGAAAG	55.0	50.0	58.1	57.4	458-473 bp	1.0	80.0