Hotspot

trnS-GGA-trnL-UAA

LSC · 126728-128184 · 1457 bp

Back to Elaphoglossum

Pi 0.0211

MarkerSeek score 60.40

Primer available yes

Label trnS-GGA-trnL-UAA

Feature Metadata

label_name: trnS-GGA-trnL-UAA
feature_type: igs
region: LSC
length_bp: 1457
variable_sites: 46
pi: 0.0211
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-trnL-UAA_p1	GTTTAAGGCATAGCACCGGA	CTACCATTCCGCCATATCCC	50.0	55.0	58.0	57.6	1548-1731 bp	1.0	79.1
trnS-GGA-trnL-UAA_p2	CTACCGAGGGTTCAAATCCC	CTACCATTCCGCCATATCCC	55.0	55.0	57.7	57.6	1508-1691 bp	1.0	76.8
trnS-GGA-trnL-UAA_p3	GCTACCGAGGGTTCAAATCC	CTACCATTCCGCCATATCCC	55.0	55.0	58.3	57.6	1509-1692 bp	1.0	76.7
trnS-GGA-trnL-UAA_p4	GAGCGGTTTAAGGCATAGCA	CTACCATTCCGCCATATCCC	50.0	55.0	58.3	57.6	1553-1736 bp	1.0	76.6
trnS-GGA-trnL-UAA_p5	GTTTAAGGCATAGCACCGGA	CGTCTACCATTCCGCCATAT	50.0	50.0	58.0	57.3	1551-1734 bp	1.0	76.4