Hotspot

trnT-GGU-trnfM-CAU

LSC · 118540-118962 · 423 bp

Back to Elaphoglossum

Pi 0.0255

MarkerSeek score 65.30

Primer available yes

Label trnT-GGU-trnfM-CAU

Feature Metadata

label_name: trnT-GGU-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 423
variable_sites: 15
pi: 0.0255
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnfM-CAU_p1	ACGACTTACGCCTTACCATG	CAGGTTCAAATCCTGTCCCC	50.0	55.0	57.7	58.2	496-594 bp	1.0	81.1
trnT-GGU-trnfM-CAU_p2	ACGACTTACGCCTTACCATG	GTAGCTCGCAAGGCTCATAA	50.0	50.0	57.7	57.8	527-625 bp	1.0	80.3
trnT-GGU-trnfM-CAU_p3	ACGACTTACGCCTTACCATG	TAGCTCGCAAGGCTCATAAC	50.0	50.0	57.7	57.8	526-624 bp	1.0	80.3
trnT-GGU-trnfM-CAU_p4	CGACTTACGCCTTACCATGG	CAGGTTCAAATCCTGTCCCC	55.0	55.0	58.4	58.2	495-593 bp	1.0	79.9
trnT-GGU-trnfM-CAU_p5	ACGACTTACGCCTTACCATG	TGAAGGCATCAACTAAGCGG	50.0	50.0	57.7	58.3	561-659 bp	1.0	79.8