Hotspot

trnC-GCA-trnG-GCC

LSC · 112947-113427 · 481 bp

Back to Elaphoglossum

Pi 0.0299

MarkerSeek score 71.40

Primer available yes

Label trnC-GCA-trnG-GCC

Feature Metadata

label_name: trnC-GCA-trnG-GCC
feature_type: igs
region: LSC
length_bp: 481
variable_sites: 21
pi: 0.0299
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-trnG-GCC_p1	TGCAGTCCTACGTCTTACCA	TCTCTTTGCCAAGGAGATGG	50.0	50.0	58.1	57.2	562-589 bp	1.0	78.1
trnC-GCA-trnG-GCC_p2	TTAGGCGACACCCAGATTTG	TCTCTTTGCCAAGGAGATGG	50.0	50.0	57.9	57.2	601-628 bp	1.0	77.6
trnC-GCA-trnG-GCC_p3	GTTAGGCGACACCCAGATTT	TCTCTTTGCCAAGGAGATGG	50.0	50.0	57.9	57.2	602-629 bp	1.0	77.6
trnC-GCA-trnG-GCC_p4	GCAGTCCTACGTCTTACCAC	TCTCTTTGCCAAGGAGATGG	55.0	50.0	57.7	57.2	561-588 bp	1.0	76.6
trnC-GCA-trnG-GCC_p5	AAGAGTCTTGTCGTTAGGCG	TCTCTTTGCCAAGGAGATGG	50.0	50.0	57.7	57.2	614-641 bp	1.0	75.6