Hotspot

psbM-trnC-GCA

LSC · 108370-112874 · 4505 bp

Back to Elaphoglossum

Pi 0.0336

MarkerSeek score 69.90

Primer available yes

Label psbM-trnC-GCA

Feature Metadata

label_name: psbM-trnC-GCA
feature_type: igs
region: LSC
length_bp: 4505
variable_sites: 214
pi: 0.0336
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnC-GCA_p1	CTCCCGTAAGTTTGCCTACC	CAGACATACCGTTGAGGGTG	55.0	55.0	58.0	58.0	861 bp	0.333333	53.1
psbM-trnC-GCA_p2	CTCCCGTAAGTTTGCCTACC	CTCACCGGTTTGAGATGAGG	55.0	55.0	58.0	58.0	912 bp	0.333333	53.0
psbM-trnC-GCA_p3	CTCCCGTAAGTTTGCCTACC	CATTCTCAGGCTGACTCACC	55.0	55.0	58.0	58.0	926 bp	0.333333	53.0
psbM-trnC-GCA_p4	CTCCCGTAAGTTTGCCTACC	TCAATAGTTGCGATGCTGCT	55.0	45.0	58.0	58.0	754 bp	0.333333	52.9
psbM-trnC-GCA_p5	CTCCCGTAAGTTTGCCTACC	TTCTCACCCTGCCAGACATA	55.0	50.0	58.0	58.0	873 bp	0.333333	52.8