Hotspot

trnG-UCC-trnR-UCU

LSC · 89958-90103 · 146 bp

Back to Elaphoglossum

Pi 0.032

MarkerSeek score 72.60

Primer available yes

Label trnG-UCC-trnR-UCU

Feature Metadata

label_name: trnG-UCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 146
variable_sites: 7
pi: 0.032
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-UCU_p1	TGTTCGGTTCTGGATTAGCG	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	273-283 bp	1.0	68.4
trnG-UCC-trnR-UCU_p2	TGTTCGGTTCTGGATTAGCG	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	279-289 bp	1.0	67.6
trnG-UCC-trnR-UCU_p3	TGTTCGGTTCTGGATTAGCG	ACCACGTTCAATTCATCTACGT	50.0	40.9	58.0	58.1	357-367 bp	1.0	67.1
trnG-UCC-trnR-UCU_p4	ACTTCCATGTTCGGTTCTGG	AGAAGACCTCTGTCCTATCCA	50.0	47.6	57.8	56.9	280-290 bp	1.0	66.9
trnG-UCC-trnR-UCU_p5	TGTTCGGTTCTGGATTAGCG	ACGCTCAATAGGATTTGAACCT	50.0	40.9	58.0	57.8	308-318 bp	1.0	66.5