Hotspot

ndhF-trnL-UAG

SSC · 115848-117222 · 1375 bp

Back to Elaeocarpus

Pi 0.0143

MarkerSeek score 60.70

Primer available yes

Label ndhF-trnL-UAG

Feature Metadata

label_name: ndhF-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1375
variable_sites: 52
pi: 0.0143
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-trnL-UAG_p1	AGCCCCGCTCCTATTAACAT	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.6	58.0	1208-1567 bp	1.0	82.2
ndhF-trnL-UAG_p2	AGCCCCGCTCCTATTAACAT	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.6	58.0	1209-1568 bp	1.0	82.2
ndhF-trnL-UAG_p3	TTTTGTTGCCGTCGGAAAAA	AGCGTGTCTACCGATTTCAC	40.0	50.0	57.4	58.0	1233-1592 bp	1.0	81.5
ndhF-trnL-UAG_p4	TTTTTGTTGCCGTCGGAAAA	AGCGTGTCTACCGATTTCAC	40.0	50.0	57.4	58.0	1234-1593 bp	1.0	81.5
ndhF-trnL-UAG_p5	TTTTGTTGCCGTCGGAAAAA	CAGCGTGTCTACCGATTTCA	40.0	50.0	57.4	58.0	1234-1593 bp	1.0	81.5