Hotspot

trnN-GUU-ndhF

IRb · 112061-113873 · 1813 bp

Back to Elaeocarpus

Pi 0.0146

MarkerSeek score 56.30

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 1813
variable_sites: 65
pi: 0.0146
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	GGCAGAATAGCAGAGCAAGT	AGTTTGTAACAAACGCAGCG	50.0	45.0	58.0	57.9	2368-2854 bp	1.0	85.0
trnN-GUU-ndhF_p2	GGCAGAATAGCAGAGCAAGT	TTGTTCGTTGGAGCCATAGG	50.0	50.0	58.0	57.9	2498-2984 bp	1.0	84.8
trnN-GUU-ndhF_p3	GGCAGAATAGCAGAGCAAGT	TTCCACAGCAGGATTAACCG	50.0	50.0	58.0	57.9	2868-2926 bp	0.555556	65.8
trnN-GUU-ndhF_p4	GGCAGAATAGCAGAGCAAGT	ATAGCTTGTTCCACAGCAGG	50.0	50.0	58.0	57.9	2876-2934 bp	0.555556	65.7
trnN-GUU-ndhF_p5	GGCAGAATAGCAGAGCAAGT	ACAGCAGGATTAACCGCATT	50.0	45.0	58.0	57.9	2864-2922 bp	0.555556	65.7