Hotspot

trnE-UUC-trnT-GGU

LSC · 32746-33418 · 673 bp

Back to Elaeocarpus

Pi 0.0151

MarkerSeek score 55.10

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 673
variable_sites: 26
pi: 0.0151
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	GCATCGACCCAGGAAGAATT	GAACCGATGACTTACGCCTT	50.0	50.0	58.0	58.0	830-877 bp	1.0	86.3
trnE-UUC-trnT-GGU_p2	GATTAGAGTCGCCGTGTTGT	GAACCGATGACTTACGCCTT	50.0	50.0	58.0	58.0	1209-1258 bp	1.0	85.6
trnE-UUC-trnT-GGU_p3	GATTAGAGTCGCCGTGTTGT	CCATGGCGTTACTCTACCAC	50.0	55.0	58.0	58.1	1188-1237 bp	1.0	85.3
trnE-UUC-trnT-GGU_p4	GATTAGAGTCGCCGTGTTGT	TGGCGTTACTCTACCACTGA	50.0	50.0	58.0	58.1	1185-1234 bp	1.0	85.1
trnE-UUC-trnT-GGU_p5	TTGGATTTTGGTCTGTCGGG	GAACCGATGACTTACGCCTT	50.0	50.0	58.1	58.0	1379-1418 bp	0.777778	75.1