Hotspot

petN-psbM

LSC · 30242-30978 · 737 bp

Back to Elaeocarpus

Pi 0.00392

MarkerSeek score 62.00

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 737
variable_sites: 10
pi: 0.00392
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAATCTCGCCTGGGCT	TGCTACTGCACTGTTCATTT	50.0	40.0	58.2	55.6	843-899 bp	1.0	63.9
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTT	50.0	40.0	57.6	55.6	782-838 bp	1.0	62.8
petN-psbM_p3	TAGTAAATCTCGCCTGGGCT	TGCTACTGCACTGTTCATTTT	50.0	38.1	58.2	56.4	843-899 bp	1.0	62.2
petN-psbM_p4	CCTGGGCTGCTTTAATGGTA	TGCTACTGCACTGTTCATTT	50.0	40.0	57.6	55.6	831-887 bp	1.0	62.1
petN-psbM_p5	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTTT	50.0	38.1	57.6	56.4	782-838 bp	1.0	61.1