Hotspot

atpF-atpH

LSC · 13522-14233 · 712 bp

Back to Elaeocarpus

Pi 0.0178

MarkerSeek score 52.90

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 712
variable_sites: 26
pi: 0.0178
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	GGGATCATGCACCTTTTCCT	ATTTCTGCCGCTTCCGTTAT	50.0	45.0	57.9	58.0	1047-1175 bp	1.0	84.9
atpF-atpH_p2	GGGATCATGCACCTTTTCCT	CGACTGGATGAATCTTGGCA	50.0	50.0	57.9	58.0	1155-1283 bp	1.0	84.8
atpF-atpH_p3	CATGCTGCTTCTGGACTTCA	ATTTCTGCCGCTTCCGTTAT	50.0	45.0	58.2	58.0	1206-1334 bp	1.0	84.3
atpF-atpH_p4	CATGCTGCTTCTGGACTTCA	CGACTGGATGAATCTTGGCA	50.0	50.0	58.2	58.0	1314-1442 bp	1.0	84.2
atpF-atpH_p5	GGGATCATGCACCTTTTCCT	AAGCCGTAGAAGGTATTGCG	50.0	50.0	57.9	58.1	1086 bp	0.111111	42.7