Hotspot

matK-trnK-UUU

LSC · 3625-4401 · 777 bp

Back to Elaeocarpus

Pi 0.016

MarkerSeek score 54.20

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 777
variable_sites: 17
pi: 0.016
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	GGAAGTCATGTTGCCGAGAT	AACGGTAGAGTACTCGGCTT	50.0	50.0	58.0	58.2	833-872 bp	1.0	82.9
matK-trnK-UUU_p2	GGAAGTCATGTTGCCGAGAT	ACGGTAGAGTACTCGGCTTT	50.0	50.0	58.0	58.2	832-871 bp	1.0	82.9
matK-trnK-UUU_p3	GGAAGTCATGTTGCCGAGAT	TTGGGTTGCTAACTCAACGG	50.0	50.0	58.0	58.4	848-887 bp	1.0	81.0
matK-trnK-UUU_p4	GGAAGTCATGTTGCCGAGAT	GGGTTGCTAACTCAACGGTA	50.0	50.0	58.0	57.5	846-885 bp	1.0	80.5
matK-trnK-UUU_p5	TAGGAAGTCATGTTGCCGAG	ACGGTAGAGTACTCGGCTTT	50.0	50.0	57.3	58.2	834-873 bp	1.0	77.8