Hotspot

trnH-GUG-psbA

LSC · 102-467 · 366 bp

Back to Elaeocarpus

Pi 0.0215
MarkerSeek score 59.20
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
366
variable_sites
20
pi
0.0215
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.98
markerseek_score
59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG CCTCTAGACCTAGCTGCTGT 55.0 55.0 58.1 57.9 423-451 bp 1.0 85.7
trnH-GUG-psbA_p2 GATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 55.0 55.0 58.1 57.9 426-454 bp 1.0 85.6
trnH-GUG-psbA_p3 CAATCCACTGCCTTGATCCA CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 437-465 bp 1.0 85.5
trnH-GUG-psbA_p4 AATCCACTGCCTTGATCCAC CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 436-464 bp 1.0 85.5
trnH-GUG-psbA_p5 ACAATCCACTGCCTTGATCC CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 438-466 bp 1.0 85.4