Hotspot

ndhA

SSC · 116558-118819 · 2262 bp

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Pi 0.0119

MarkerSeek score 55.20

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2262
variable_sites: 86
pi: 0.0119
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	AGCCGAGAGAATAGCATATTCC	TCCGCCGGGTTTTATCAATT	45.5	45.0	57.5	57.9	2404-2441 bp	0.916667	61.1
ndhA_p2	GCCGAGAGAATAGCATATTCCT	TCCGCCGGGTTTTATCAATT	45.5	45.0	57.5	57.9	2403-2440 bp	0.916667	61.1
ndhA_p3	TGTTTAAGCCGAGAGAATAGCA	TCCGCCGGGTTTTATCAATT	40.9	45.0	57.5	57.9	2410-2447 bp	0.916667	61.1
ndhA_p4	GCCGAGAGAATAGCATATTCC	TCCGCCGGGTTTTATCAATT	47.6	45.0	56.1	57.9	2403-2440 bp	0.916667	57.5
ndhA_p5	GCCGAGAGAATAGCATATTCCT	GTCCGCCGGGTTTTATCAAT	45.5	50.0	57.5	58.6	2404-2441 bp	0.916667	57.3