Hotspot

rps15-ycf1

SSC · 120386-120768 · 383 bp

Back to Elaeagnus

Pi 0.0275
MarkerSeek score 66.90
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
383
variable_sites
32
pi
0.0275
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.97
markerseek_score
66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 ACTGAGATGAATGAATTTTTGACCA TCCGCGATTGATAATTCCTT 32.0 40.0 57.5 54.9 412-431 bp 1.0 50.1
rps15-ycf1_p2 ACTGAGATGAATGAATTTTTGACCA TGTATCCGCGATTGATAATTCCT 32.0 39.1 57.5 57.9 416-435 bp 1.0 50.1
rps15-ycf1_p3 ACTGAGATGAATGAATTTTTGACCA ATCCGCGATTGATAATTCCT 32.0 40.0 57.5 54.7 413-432 bp 1.0 50.1
rps15-ycf1_p4 ACTGAGATGAATGAATTTTTGACCA ATCCGCGATTGATAATTCCTT 32.0 38.1 57.5 55.5 413-432 bp 1.0 50.1
rps15-ycf1_p5 ACTGAGATGAATGAATTTTTGACCA TGTATCCGCGATTGATAATTCC 32.0 40.9 57.5 56.5 416-435 bp 1.0 50.1