Hotspot

trnH-GUG-rpl2

IRb · 81311-81408 · 98 bp

Back to Elaeagnus

Pi 0.0568

MarkerSeek score 70.30

Primer available yes

Label trnH-GUG-rpl2

Feature Metadata

label_name: trnH-GUG-rpl2
feature_type: igs
region: IRb
length_bp: 98
variable_sites: 31
pi: 0.0568
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-rpl2_p1	CGGATGTAGCCAAGTGGATC	CTTCGGCGCCGTAGTAAATA	55.0	50.0	58.1	57.9	187-205 bp	1.0	90.0
trnH-GUG-rpl2_p2	CGGATGTAGCCAAGTGGATC	TTCGGCGCCGTAGTAAATAG	55.0	50.0	58.1	57.9	186-204 bp	1.0	90.0
trnH-GUG-rpl2_p3	TGGATCAAGGCAGTGGATTG	CTTCGGCGCCGTAGTAAATA	50.0	50.0	57.9	57.9	173-191 bp	1.0	89.9
trnH-GUG-rpl2_p4	TGGATCAAGGCAGTGGATTG	TTCGGCGCCGTAGTAAATAG	50.0	50.0	57.9	57.9	172-190 bp	1.0	89.9
trnH-GUG-rpl2_p5	GGATCAAGGCAGTGGATTGT	TTCGGCGCCGTAGTAAATAG	50.0	50.0	57.9	57.9	171-189 bp	1.0	89.9