Hotspot

rpl33-rps18

LSC · 64851-65120 · 270 bp

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Pi 0.0158

MarkerSeek score 59.20

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 270
variable_sites: 15
pi: 0.0158
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	CCGGTCGATTGGAATTGAGA	TCAATTCGATCCCCCGATTG	50.0	50.0	57.7	57.7	430-475 bp	1.0	80.0
rpl33-rps18_p2	TAATAAGCCCGGTCGATTGG	TCAATTCGATCCCCCGATTG	50.0	50.0	57.5	57.7	438-483 bp	1.0	78.1
rpl33-rps18_p3	CCGGTCGATTGGAATTGAGA	ATTCGATCCCCCGATTGGAT	50.0	50.0	57.7	58.6	427-472 bp	1.0	77.0
rpl33-rps18_p4	CCCGGTCGATTGGAATTGAG	TCAATTCGATCCCCCGATTG	55.0	50.0	58.7	57.7	431-476 bp	1.0	76.9
rpl33-rps18_p5	CGACATAATAAGCCCGGTCG	TCAATTCGATCCCCCGATTG	55.0	50.0	58.6	57.7	443 bp	0.083333	36.6