Hotspot

psbE-petL

LSC · 62055-62882 · 828 bp

Back to Elaeagnus

Pi 0.0135

MarkerSeek score 61.40

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 828
variable_sites: 45
pi: 0.0135
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	GCGGAGGTTATAGTCAAAGCT	50.0	47.6	57.8	57.8	914-1023 bp	1.0	76.2
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	AGCGGAGGTTATAGTCAAAGC	50.0	47.6	57.8	57.8	915-1024 bp	1.0	76.1
psbE-petL_p3	ACGAATAACCAACCCGCAAT	GCGGAGGTTATAGTCAAAGCT	45.0	47.6	58.2	57.8	1000-1109 bp	1.0	75.6
psbE-petL_p4	CAACCCGCAATGAATAGGGA	AGCGGAGGTTATAGTCAAAGC	50.0	47.6	57.9	57.8	992-1101 bp	0.916667	72.7
psbE-petL_p5	CAACCCGCAATGAATAGGGA	GCGGAGGTTATAGTCAAAGCT	50.0	47.6	57.9	57.8	991-1100 bp	0.916667	72.7