Hotspot

petA-psbJ

LSC · 60901-61276 · 376 bp

Back to Elaeagnus

Pi 0.0217

MarkerSeek score 62.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 376
variable_sites: 26
pi: 0.0217
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCATCTGTTCTTTTGGCAC	GCTGGTATTCTTGTGATCGGT	50.0	47.6	57.9	58.1	536-575 bp	1.0	76.8
petA-psbJ_p2	GGCATCTGTTCTTTTGGCAC	AGCTGGTATTCTTGTGATCGG	50.0	47.6	57.9	57.8	537-576 bp	1.0	76.0
petA-psbJ_p3	CATTACGTGTCCAAGGCCTT	GCTGGTATTCTTGTGATCGGT	50.0	47.6	58.2	58.1	567-606 bp	1.0	75.7
petA-psbJ_p4	GACCCATTACGTGTCCAAGG	GCTGGTATTCTTGTGATCGGT	55.0	47.6	58.3	58.1	571-610 bp	1.0	75.1
petA-psbJ_p5	CATTACGTGTCCAAGGCCTT	AGCTGGTATTCTTGTGATCGG	50.0	47.6	58.2	57.8	568-607 bp	1.0	75.0