Hotspot

ycf3-trnS-GGA

LSC · 42963-43793 · 831 bp

Back to Elaeagnus

Pi 0.0149

MarkerSeek score 65.60

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 831
variable_sites: 43
pi: 0.0149
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CGAAGTTGTCGGAATTATTCGT	CCCTCGGTAAACAAAAGCCT	40.9	50.0	57.2	58.1	988-1062 bp	1.0	63.5
ycf3-trnS-GGA_p2	CGAAGTTGTCGGAATTATTCGT	ACGGAAAGAGAGGGATTCGA	40.9	50.0	57.2	58.2	1009-1083 bp	1.0	62.9
ycf3-trnS-GGA_p3	CGAAGTTGTCGGAATTATTCGT	ACCCTCGGTAAACAAAAGCC	40.9	50.0	57.2	58.4	989-1063 bp	1.0	61.1
ycf3-trnS-GGA_p4	CGAAGTTGTCGGAATTATTCGT	AGTGTACGGAAAGAGAGGGA	40.9	50.0	57.2	57.4	1014-1088 bp	1.0	59.4
ycf3-trnS-GGA_p5	CGAAGTTGTCGGAATTATTCGT	GGATTCGAACCCTCGGTAAA	40.9	50.0	57.2	57.3	997-1071 bp	1.0	58.8