Hotspot

trnE-UUC-trnT-GGU

LSC · 29427-30057 · 631 bp

Back to Elaeagnus

Pi 0.0177

MarkerSeek score 61.50

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 631
variable_sites: 41
pi: 0.0177
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	AGAGATGTCCTGAACCGCTA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	661-809 bp	1.0	87.2
trnE-UUC-trnT-GGU_p2	AGATGTCCTGAACCGCTAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	659-807 bp	1.0	87.2
trnE-UUC-trnT-GGU_p3	AGAGATGTCCTGAACCGCTA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.9	58.1	640-788 bp	1.0	86.9
trnE-UUC-trnT-GGU_p4	AGATGTCCTGAACCGCTAGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.9	58.1	638-786 bp	1.0	86.9
trnE-UUC-trnT-GGU_p5	AACTTCCTTTCGTAGCACCC	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	722-870 bp	1.0	86.7