Hotspot

rpoB-trnC-GCA

LSC · 25348-26517 · 1170 bp

Back to Elaeagnus

Pi 0.0169

MarkerSeek score 61.10

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1170
variable_sites: 72
pi: 0.0169
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CGAGCCCATATCCTTGCTTT	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.0	58.1	1724-1786 bp	1.0	86.8
rpoB-trnC-GCA_p2	TTCTGTTTCCTACTCACGCG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.9	58.1	1742-1804 bp	1.0	86.6
rpoB-trnC-GCA_p3	CGAGCCCATATCCTTGCTTT	AATCCCACCGTCTTCGATTG	50.0	50.0	58.0	58.0	2206-2262 bp	0.666667	73.4
rpoB-trnC-GCA_p4	TTCTGTTTCCTACTCACGCG	AATCCCACCGTCTTCGATTG	50.0	50.0	57.9	58.0	2224-2280 bp	0.666667	73.1
rpoB-trnC-GCA_p5	CGAGCCCATATCCTTGCTTT	TCTAATCCCACCGTCTTCGA	50.0	50.0	58.0	57.9	2209-2265 bp	0.666667	72.5