Hotspot

atpH-atpI

LSC · 12184-13063 · 880 bp

Back to Elaeagnus

Pi 0.0196

MarkerSeek score 56.10

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 880
variable_sites: 57
pi: 0.0196
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	TCTATTCAGCCCTCCCGTAG	ATTTTTGCAACCTTAGCCGC	55.0	45.0	58.0	57.9	1318-1406 bp	1.0	87.3
atpH-atpI_p2	AAGTGCTAATGCCACAACCA	CAAATTGGTCAGGGGCTCTT	45.0	50.0	58.0	58.1	1524-1615 bp	1.0	86.5
atpH-atpI_p3	TCTATTCAGCCCTCCCGTAG	CAAATTGGTCAGGGGCTCTT	55.0	50.0	58.0	58.1	1683-1771 bp	1.0	86.4
atpH-atpI_p4	AAGTGCTAATGCCACAACCA	TTCAAATTGGTCAGGGGCTC	45.0	50.0	58.0	58.1	1526-1617 bp	1.0	86.4
atpH-atpI_p5	TCTATTCAGCCCTCCCGTAG	TTCAAATTGGTCAGGGGCTC	55.0	50.0	58.0	58.1	1685-1773 bp	1.0	86.3