Hotspot

trnK-UUU-rps16

LSC · 3968-4454 · 487 bp

Back to Elaeagnus

Pi 0.0166
MarkerSeek score 56.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
487
variable_sites
37
pi
0.0166
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 TCAGGATCAGTCGTGGTCTT CTTGAGCCGTACGAGGAAAA 50.0 50.0 58.1 57.9 914-1057 bp 1.0 86.2
trnK-UUU-rps16_p2 CCTTTCAGGATCAGTCGTGG CTTGAGCCGTACGAGGAAAA 55.0 50.0 58.0 57.9 918-1061 bp 0.916667 82.9
trnK-UUU-rps16_p3 CCTTTCAGGATCAGTCGTGG TTTTCTTGAGCCGTACGAGG 55.0 50.0 58.0 57.9 922-1065 bp 0.916667 82.9
trnK-UUU-rps16_p4 CCCCGATAAGGCTCTATCCA CTTGAGCCGTACGAGGAAAA 55.0 50.0 58.1 57.9 1072-1085 bp 0.166667 50.0
trnK-UUU-rps16_p5 CCCCGATAAGGCTCTATCCA TTTTCTTGAGCCGTACGAGG 55.0 50.0 58.1 57.9 1076-1089 bp 0.166667 49.9