Hotspot

trnS-GCU

LSC · 7625-7712 · 88 bp

Back to Ehrharta

Pi 0.0568

MarkerSeek score 68.90

Primer available yes

Label trnS-GCU

Feature Metadata

label_name: trnS-GCU
feature_type: tRNA
region: LSC
length_bp: 88
variable_sites: 5
pi: 0.0568
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU_p1	GAGATAATCCGGGGGTCAGA	GAAACCTTTCAATTTGGAACGG	55.0	40.9	58.0	56.7	169 bp	1.0	55.8
trnS-GCU_p2	GAGATAATCCGGGGGTCAGA	GGAAACCTTTCAATTTGGAACG	55.0	40.9	58.0	56.7	170 bp	1.0	55.8
trnS-GCU_p3	GAGATAATCCGGGGGTCAGA	CGGAAACCTTTCAATTTGGAAC	55.0	40.9	58.0	56.7	171 bp	1.0	55.7
trnS-GCU_p4	GAGATAATCCGGGGGTCAGA	AACCTTTCAATTTGGAACGG	55.0	40.0	58.0	54.7	167 bp	1.0	55.7
trnS-GCU_p5	GAGATAATCCGGGGGTCAGA	AAACCTTTCAATTTGGAACGG	55.0	38.1	58.0	55.5	168 bp	1.0	54.3