Hotspot

psbH

LSC · 70468-70692 · 225 bp

Back to Ehrharta

Pi 0.0178

MarkerSeek score 49.20

Primer available yes

Label psbH

Feature Metadata

label_name: psbH
feature_type: gene
region: LSC
length_bp: 225
variable_sites: 4
pi: 0.0178
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbH_p1	TCTTCTTCCGAGAACCACCT	AAACAAAGGGCAAGGGTTCT	50.0	45.0	58.0	57.8	1041-1044 bp	1.0	80.0
psbH_p2	TCTTCTTCCGAGAACCACCT	AACAAAGGGCAAGGGTTCTT	50.0	45.0	58.0	57.8	1040-1043 bp	1.0	80.0
psbH_p3	ACCAGATATGGAGATGGCGA	AAACAAAGGGCAAGGGTTCT	50.0	45.0	58.0	57.8	848-851 bp	1.0	79.8
psbH_p4	ACCAGATATGGAGATGGCGA	AACAAAGGGCAAGGGTTCTT	50.0	45.0	58.0	57.8	847-850 bp	1.0	79.8
psbH_p5	ACTACAAGGAGACAGGCAGT	AACAAAGGGCAAGGGTTCTT	50.0	45.0	58.0	57.8	1306-1309 bp	1.0	79.8