Hotspot

psbT-psbN

LSC · 70180-70232 · 53 bp

Back to Ehrharta

Pi 0.0943

MarkerSeek score 77.90

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 53
variable_sites: 5
pi: 0.0943
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	TTCCGAGAACCACCTAAGGT	ATCCATTCGAAGAACACGGG	50.0	50.0	58.0	58.0	121 bp	1.0	83.0
psbT-psbN_p2	TCTTCTTCCGAGAACCACCT	ATCCATTCGAAGAACACGGG	50.0	50.0	58.0	58.0	126 bp	1.0	82.9
psbT-psbN_p3	TTCCGAGAACCACCTAAGGT	TCGAAGAACACGGGGACTAA	50.0	50.0	58.0	58.4	115 bp	1.0	80.3
psbT-psbN_p4	TCTTCTTCCGAGAACCACCT	TCGAAGAACACGGGGACTAA	50.0	50.0	58.0	58.4	120 bp	1.0	80.2
psbT-psbN_p5	TCTTCTTCCGAGAACCACCT	TTCGAAGAACACGGGGACTA	50.0	50.0	58.0	58.4	121 bp	1.0	80.2