Hotspot

rbcL-psaI

LSC · 55230-56904 · 1675 bp

Back to Ehrharta

Pi 0.021

MarkerSeek score 58.90

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 1675
variable_sites: 30
pi: 0.021
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	GGCTTCAGGGGGTATTCATG	GTCATTGCAATTGCCGGAAA	55.0	45.0	58.0	57.9	1847-2050 bp	1.0	84.6
rbcL-psaI_p2	GAGCTGTATTTGCGAGGGAA	GTCATTGCAATTGCCGGAAA	50.0	45.0	58.0	57.9	2220-2423 bp	1.0	84.4
rbcL-psaI_p3	GGCTTCAGGGGGTATTCATG	CGCGATTCCGTAGAAAGACA	55.0	50.0	58.0	58.1	2465-2668 bp	1.0	84.4
rbcL-psaI_p4	GGCTTCAGGGGGTATTCATG	GGAGCTAATGAATAGGCCCG	55.0	55.0	58.0	57.9	2483-2686 bp	1.0	84.4
rbcL-psaI_p5	GGCTTCAGGGGGTATTCATG	CGCATCCATAGCCGTTTTTC	55.0	50.0	58.0	57.9	2018-2221 bp	1.0	84.4