Hotspot

trnF-GAA-ndhJ

LSC · 47129-47639 · 511 bp

Back to Ehrharta

Pi 0.00992

MarkerSeek score 59.70

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 511
variable_sites: 5
pi: 0.00992
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	AAAGTTGGATAGGTTGGCCC	50.0	50.0	58.1	57.8	619-626 bp	1.0	79.3
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	TCCACGCCTTAAACGTATCC	50.0	50.0	58.1	57.7	648-655 bp	1.0	78.7
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	CCACGCCTTAAACGTATCCT	50.0	50.0	58.1	57.7	647-654 bp	1.0	78.6
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	ATCATCCACGCCTTAAACGT	50.0	45.0	58.1	57.6	652-659 bp	1.0	77.9
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGTTGGCCCTT	50.0	50.0	58.1	58.6	617-624 bp	1.0	76.2