Hotspot

rpoC2-rps2

LSC · 29566-29878 · 313 bp

Back to Ehrharta

Pi 0.0315

MarkerSeek score 62.00

Primer available yes

Label rpoC2-rps2

Feature Metadata

label_name: rpoC2-rps2
feature_type: igs
region: LSC
length_bp: 313
variable_sites: 9
pi: 0.0315
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2-rps2_p1	CAGAATCACCATTTACCCCCA	TGATGTTCCAATATCTTCTTGTCA	47.6	33.3	57.6	56.1	346-373 bp	1.0	46.0
rpoC2-rps2_p2	TCAGAATCACCATTTACCCCC	TGATGTTCCAATATCTTCTTGTCA	47.6	33.3	57.3	56.1	347-374 bp	1.0	46.0
rpoC2-rps2_p3	CAGAATCACCATTTACCCCCA	TTGATGTTCCAATATCTTCTTGTCA	47.6	32.0	57.6	56.8	347-374 bp	1.0	46.0
rpoC2-rps2_p4	CAGAATCACCATTTACCCCCA	TGATGTTCCAATATCTTCTTGTCAT	47.6	32.0	57.6	56.6	346-373 bp	1.0	46.0
rpoC2-rps2_p5	AGAATCACCATTTACCCCCA	TGATGTTCCAATATCTTCTTGTCA	45.0	33.3	56.1	56.1	345-372 bp	1.0	46.0