Hotspot

rpoB-rpoC1

LSC · 22717-22753 · 37 bp

Back to Ehrharta

Pi 0.027

MarkerSeek score 65.80

Primer available yes

Label rpoB-rpoC1

Feature Metadata

label_name: rpoB-rpoC1
feature_type: igs
region: LSC
length_bp: 37
variable_sites: 1
pi: 0.027
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-rpoC1_p1	TCGTCCGAGAACTACGATCT	CCAACGACTTCCCCATTAGG	50.0	55.0	57.7	58.0	230 bp	1.0	77.6
rpoB-rpoC1_p2	TCGTCCGAGAACTACGATCT	TATTTGTTGCGGGGAAACGA	50.0	45.0	57.7	58.1	186 bp	1.0	77.1
rpoB-rpoC1_p3	CTCGTCCGAGAACTACGATC	CCAACGACTTCCCCATTAGG	55.0	55.0	57.4	58.0	231 bp	1.0	74.8
rpoB-rpoC1_p4	CTCGTCCGAGAACTACGATC	TATTTGTTGCGGGGAAACGA	55.0	45.0	57.4	58.1	187 bp	1.0	74.4
rpoB-rpoC1_p5	TCGTCCGAGAACTACGATCT	CGGGGAAACGAGTCCAATTT	50.0	50.0	57.7	58.5	177 bp	1.0	74.2