Hotspot

trnD-GUC-psbM

LSC · 15860-16633 · 774 bp

Back to Ehrharta

Pi 0.0228

MarkerSeek score 70.80

Primer available yes

Label trnD-GUC-psbM

Feature Metadata

label_name: trnD-GUC-psbM
feature_type: igs
region: LSC
length_bp: 774
variable_sites: 17
pi: 0.0228
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbM_p1	ATTTAAGAATCACCCCGCGG	TCATTTTGGCTGGCTGTTTT	50.0	40.0	58.3	56.7	950-968 bp	1.0	70.8
trnD-GUC-psbM_p2	TTCAATTGGTCAGAGCACCG	TCATTTTGGCTGGCTGTTTT	50.0	40.0	58.5	56.7	921-939 bp	1.0	69.7
trnD-GUC-psbM_p3	ATTTAAGAATCACCCCGCGG	AATCATTTTGGCTGGCTGTT	50.0	40.0	58.3	56.5	952-970 bp	1.0	68.9
trnD-GUC-psbM_p4	ATTTAAGAATCACCCCGCGG	ATCATTTTGGCTGGCTGTTT	50.0	40.0	58.3	56.5	951-969 bp	1.0	68.9
trnD-GUC-psbM_p5	ATTTAAGAATCACCCCGCGG	TCATTTTGGCTGGCTGTTTTT	50.0	38.1	58.3	57.4	950-968 bp	1.0	68.7