Hotspot

psbK-psbI

LSC · 6997-7393 · 397 bp

Back to Ehrharta

Pi 0.0252

MarkerSeek score 58.00

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 397
variable_sites: 10
pi: 0.0252
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	TCCAAAGATAAAGAGGGAAACA	50.0	36.4	57.9	55.0	493 bp	1.0	41.6
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TCCAAAGATAAAGAGGGAAACAA	50.0	34.8	57.9	55.7	493 bp	1.0	41.6
psbK-psbI_p3	TGGCAAGCTGCTGTAAGTTT	TCCAAAGATAAAGAGGGAAACA	45.0	36.4	58.3	55.0	490 bp	1.0	41.6
psbK-psbI_p4	TTGGCAAGCTGCTGTAAGTT	TCCAAAGATAAAGAGGGAAACA	45.0	36.4	58.3	55.0	491 bp	1.0	41.6
psbK-psbI_p5	TTTGGCAAGCTGCTGTAAGT	TCCAAAGATAAAGAGGGAAACA	45.0	36.4	58.3	55.0	492 bp	1.0	41.6