Hotspot

ycf1

SSC · 126288-131732 · 5445 bp

Back to Ehretia

Pi 0.0115

MarkerSeek score 48.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5445
variable_sites: 94
pi: 0.0115
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCCGTCCCCCTTGTAAAATA	AACCCCTTCGTCTGTCTGTA	50.0	50.0	56.9	58.0	323 bp	1.0	73.6
ycf1_p2	CCCGTCCCCCTTGTAAAATA	ACCCCTTCGTCTGTCTGTAA	50.0	50.0	56.9	58.0	322 bp	1.0	73.6
ycf1_p3	CCCGTCCCCCTTGTAAAATA	CCGTGGGTTATACCAAGCAA	50.0	50.0	56.9	57.9	855 bp	1.0	72.1
ycf1_p4	CCCGTCCCCCTTGTAAAATA	AACCGTGGGTTATACCAAGC	50.0	50.0	56.9	57.9	857 bp	1.0	72.1
ycf1_p5	CCCGTCCCCCTTGTAAAATA	TCCTCGATTCAACGGAGAGA	50.0	50.0	56.9	57.9	420 bp	1.0	72.1