Hotspot

rpoC2

LSC · 17137-21282 · 4146 bp

Back to Ehretia

Pi 0.00611

MarkerSeek score 46.00

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4146
variable_sites: 38
pi: 0.00611
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	TGAAAGGGATGAGTCTGCTC	GTTGGTACCGGATTCAAAGGA	50.0	47.6	56.9	57.9	209 bp	1.0	65.6
rpoC2_p2	GGGATGAGTCTGCTCTTAGG	GTTGGTACCGGATTCAAAGGA	55.0	47.6	56.8	57.9	204 bp	1.0	64.7
rpoC2_p3	AGGGATGAGTCTGCTCTTAGG	GTTGGTACCGGATTCAAAGGA	52.4	47.6	58.3	57.9	205 bp	1.0	63.6
rpoC2_p4	GGGATGAGTCTGCTCTTAGGA	GTTGGTACCGGATTCAAAGGA	52.4	47.6	58.3	57.9	204 bp	1.0	63.6
rpoC2_p5	TTGAAAGGGATGAGTCTGCTC	GTTGGTACCGGATTCAAAGGA	47.6	47.6	57.7	57.9	210 bp	1.0	63.4